Heterologous expression of C. elegans ion channels in Xenopus oocytes
نویسندگان
چکیده
منابع مشابه
Heterologous expression of C. elegans ion channels in Xenopus oocytes.
Physiological methods entered the world of C. elegans, a model system used for many years to study development and a plethora of biological processes mainly employing genetic, molecular and anatomical techniques. One of the methods introduced by physiologists is the use of Xenopus oocytes for expression of C. elegans ion channels. Oocytes of the South African frog Xenopus laevis are used widely...
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Xenopus oocytes have been widely used for studying ion channels in a controlled in vivo environment since the system was initially developed for this purpose by Miledi and coworkers [1, 2]. There have been at least five major types of studies using oocytes to examine ion channel expression. The earliest use was to examine the properties of specific ion channels in a living cell free from other ...
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Injection of Caenorhabditis elegans polyA RNA into Xenopus laevis oocytes led to the expression of neurotransmitter receptors that generated some unique responses, including ionotropic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors as well as receptors that coupled to G proteins, such as those to octopamine, norepinephrine, and angiotensin, which activated the oocyte's own p...
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Muscarinic potassium channels (KAch) are composed of two subunits, GIRK1 and GIRK4 (or CIR), and are directly gated by G proteins. We have identified a novel gating mechanism of KAC h, independent of G-protein activation. This mechanism involved functional modification of KACh which required hydrolysis of physiological levels of intracellular ATP and was manifested by an increase in the channel...
متن کاملPatch clamp recording of ion channels expressed in Xenopus oocytes.
Since its development by Sakmann and Neher (1, 2), the patch clamp has become established as an extremely useful technique for electrophysiological measurement of single or multiple ion channels in cells. This technique can be applied to ion channels in both their native environment and expressed in heterologous cells, such as oocytes harvested from the African clawed frog, Xenopus laevis. Here...
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ژورنال
عنوان ژورنال: WormBook
سال: 2006
ISSN: 1551-8507
DOI: 10.1895/wormbook.1.117.1